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Typically, E3s recognize substrates that are modified, often by phosphorylation (for more details, read Zheng & Shabek and Shabek & Zheng and Shabek & Ciechanover)

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The concentrationresponse curves (Fig

The best performance can be achieved using ultra-high-performance chromatography (increased resolution and shortened time of analysis in comparison to regular HPLC), a diode array detector (measurement of absorption at various wavelengths is useful to detect molecules differing in absorption maxima

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